Nature communications, 2019-02, Vol.10 (1), p.967-967, Article 967
2019
Details
- Autor(en) / Beteiligte
- Titel
- Perivascular cell-specific knockout of the stem cell pluripotency gene Oct4 inhibits angiogenesis
- Ist Teil von
- Nature communications, 2019-02, Vol.10 (1), p.967-967, Article 967
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The stem cell pluripotency factor Oct4 serves a critical protective role during atherosclerotic plaque development by promoting smooth muscle cell (SMC) investment. Here, we show using Myh11-CreER lineage-tracing with inducible SMC and pericyte (SMC-P) knockout of Oct4 that Oct4 regulates perivascular cell migration and recruitment during angiogenesis. Knockout of Oct4 in perivascular cells significantly impairs perivascular cell migration, increases perivascular cell death, delays endothelial cell migration, and promotes vascular leakage following corneal angiogenic stimulus. Knockout of Oct4 in perivascular cells also impairs perfusion recovery and decreases angiogenesis following hindlimb ischemia. Transcriptomic analyses demonstrate that expression of the migratory gene Slit3 is reduced following loss of Oct4 in cultured SMCs, and in Oct4-deficient perivascular cells in ischemic hindlimb muscle. Together, these results provide evidence that Oct4 plays an essential role within perivascular cells in injury- and hypoxia-induced angiogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-019-08811-zTitel-ID: cdi_doaj_primary_oai_doaj_org_article_e55a3bf1dfe040a6ab7dfaf6ab1c2344
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Arteriosclerosis, Atherosclerosis, Cell adhesion & migration, Cell Death, Cell Lineage, Cell migration, Cell Movement, Cells, Cultured, Cornea, Corneal Neovascularization - metabolism, Corneal Neovascularization - pathology, Endothelial cells, Female, Gene expression, Hindlimb, Hypoxia, Ischemia, Ischemia - metabolism, Ischemia - pathology, Male, Membrane Proteins - metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Muscles, Myocytes, Smooth Muscle - metabolism, Neovascularization, Pathologic, Neovascularization, Physiologic, Oct-4 protein, Octamer Transcription Factor-3 - deficiency, Octamer Transcription Factor-3 - genetics, Perfusion, Pericytes - metabolism, Pericytes - pathology, Pluripotency, Pluripotent Stem Cells - metabolism, Pluripotent Stem Cells - pathology, Smooth muscle, Stem cells