Details

Autor(en) / Beteiligte

• Creutzenberg, Otto
• Hammann, Volker
• Wolf, Stefanie
• Daul, Jürgen
• Ngiewih, Yufanyi
• Chaudhuri, Ishrat
• Levy, Len

Titel

Toxicokinetic study following intratracheal instillation or oral gavage of two [7Be]-tagged carbon black samples

Ist Teil von

• Particle and fibre toxicology, 2022-10, Vol.19 (1), p.1-63, Article 63

Ort / Verlag

London: BioMed Central Ltd

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• The toxicokinetic behaviour of nanostructured particles following pulmonary or oral deposition is of great scientific interest. In this toxicokinetic study, following the general principles of OECD TG 417, the systemic availability of carbon black, a nanostructured material consisting of agglomerated aggregates was characterised. Each of two grades of beryllium-7 labelled carbon black (Monarch[R] 1000, oxidized and Printex[R] 90; untreated) was administered either intratracheally or orally to adult rats. Independent of route, rats received a single dose of approximately 0.3 mg radiolabelled carbon black. A total of 12 rats were treated per grade and per exposure route: 4 females each for feces/urine/organs and serial blood kinetics; 4 males for organs. At necropsy, the complete suite of organs was analysed for females, but only the lungs, liver, kidney, reproductive organs for males. In the pulmonarily exposed animals, .sup.7Be-Monarch[R] 1000 and .sup.7Be-Printex[R] 90 was detected in feces in the first 3 days after treatment at significant levels, i.e. 17.6% and 8.2%, respectively. In urine, small percentages of 6.7% and 0.4% were observed, respectively. In blood, radioactivity, representative of carbon black was within the background noise of the measurement method. At necropsy, 20 days post-instillation, both test items were practically exclusively found in lungs (75.1% and 91.0%, respectively) and in very small amounts (approximately 0.5%) in the lung-associated lymph nodes (LALN). In the other organs/tissues the test item was not detectable. BAL analyses indicated that carbon black particles were completely engulfed by alveolar macrophages. Radioactivity, representative of carbon black, was not detected beyond the experimentally defined limit of quantitation systemically after deposition in lungs or stomach in rats. Under these experimental conditions, the two CB samples were not shown to translocate beyond the lung or the GI tract into the blood compartment.