Details

Autor(en) / Beteiligte

• Srivastava, Mayank
• Zhang, Ying
• Chen, Jian
• Sirohi, Devika
• Miller, Andrew
• Zhang, Yang
• Chen, Zhilu
• Lu, Haojie
• Xu, Jianqing
• Kuhn, Richard J
• Andy Tao, W

Titel

Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor

Ist Teil von

• Nature communications, 2020-08, Vol.11 (1), p.3896-3896, Article 3896

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The outbreak of Zika virus (ZIKV) in 2016 created worldwide health emergency which demand urgent research efforts on understanding the virus biology and developing therapeutic strategies. Here, we present a time-resolved chemical proteomic strategy to track the early-stage entry of ZIKV into host cells. ZIKV was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on UV exposure at different time points, and a biotin tag for subsequent enrichment and mass spectrometric identification of the receptor or other host proteins critical for virus internalization. We identified Neural Cell Adhesion Molecule (NCAM1) as a potential ZIKV receptor and further validated it through overexpression, knockout, and inhibition of NCAM1 in Vero cells and human glioblastoma cells U-251 MG. Collectively, the strategy can serve as a universal tool to map virus entry pathways and uncover key interacting proteins.