International journal of molecular sciences, 2022-03, Vol.23 (7), p.3510
2022
Details
- Autor(en) / Beteiligte
- Titel
- Mesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis
- Ist Teil von
- International journal of molecular sciences, 2022-03, Vol.23 (7), p.3510
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2022
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how , one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO-α, RANTES, TLR-2, HIF-1α, OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO-α were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF-β, LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by treatment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms23073510Titel-ID: cdi_doaj_primary_oai_doaj_org_article_dd763e14b32840fda08de017c58defc8
- Format
- –
- Schlagworte
- Adipocytes, Antigens, Bacteria, Biomedical materials, Bone marrow, Cell culture, cell differentiation, Cell Differentiation - genetics, Cell proliferation, Cell size, Cells, Cultured, Chemokine CCL5 - metabolism, Chemokine CXCL10 - metabolism, Chondroblasts, Culture, Cytokines, Etiology, Gene expression, Gingiva, Gingiva-Derived Mesenchymal Stromal Cells, Humans, Hypoxia-inducible factor 1a, Inflammation, Inflammatory diseases, Insulin-like growth factor-binding protein 4, Interleukin 6, Interleukin 8, Interleukin-6 - metabolism, IP-10 protein, Ligaments, Mesenchymal Stem Cells - metabolism, Mesenchyme, Monocyte chemoattractant protein 1, Morphology, Osteoprotegerin, Periodontitis, Periodontitis - metabolism, Porphyromonas gingivalis, Proteins, RANTES, SDF-1 protein, Stem cells, Stromal cells, Tissue engineering, Tissue inhibitor of metalloproteinase 1, tissue regeneration, TLR2 protein, Toll-like receptors, Virulence