Details

Autor(en) / Beteiligte

• Haumaier, Franziska
• Schneider-Fuchs, Anna
• Backert, Steffen
• Vieth, Michael
• Sterlacci, William
• Wöhrl, Birgitta M

Titel

Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

Ist Teil von

• Pathogens (Basel), 2022-01, Vol.11 (1), p.59

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• The treatment of infections by the gastric pathogen ( ) has become more difficult due to increased rates of resistances against various antibiotics. Typically, atriple therapy, employing a combination of at least two antibiotics and a proton pump inhibitor, is used to cure infections. In case of first-line therapy failure, quinolones are commonly applied in a second-line therapy. To prevent second-line treatment failures, we developed an improved method to detect the most common quinolone-resistance mutations located in the quinolone-resistance-determining region (QRDR) of the bacterial gene. Biopsy material from the gastric mucosa of infected patients was used to identify quinolone-resistant strains before the onset of drug administration. Two different wild-type and six mutant QRDR sequences were included. Melting curve analyses were performed with corresponding plasmid DNAs using a real-time polymerase chain reaction (RT-PCR) assay. By applying a combination of only two different fluorescent probes, this assay allows wild-type sequences to be unambiguously distinguished from all known mutant QRDR sequences of . Next, the T values of patient DNAs were established, and the genotypes were confirmed by sequencing. Thus, quinolone-resistant strains can be easily and quickly diagnosed before treatment, which will help to avoid the administration of ineffective drug regimes.