International journal of molecular sciences, 2022-02, Vol.23 (4), p.2147
2022
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Details
- Autor(en) / Beteiligte
- Titel
- Detection of Pathological Markers of Neurodegenerative Diseases following Microfluidic Direct Conversion of Patient Fibroblasts into Neurons
- Ist Teil von
- International journal of molecular sciences, 2022-02, Vol.23 (4), p.2147
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease are clinically diagnosed using neuropsychological and cognitive tests, expensive neuroimaging-based approaches (MRI and PET) and invasive and time-consuming lumbar puncture for cerebrospinal fluid (CSF) sample collection to detect biomarkers. Thus, a rapid, simple and cost-effective approach to more easily access fluids and tissues is in great need. Here, we exploit the chemical direct reprogramming of patient skin fibroblasts into neurons (chemically induced neurons, ciNs) as a novel strategy for the rapid detection of different pathological markers of neurodegenerative diseases. We found that FAD fibroblasts have a reduced efficiency of reprogramming, and converted ciNs show a less complex neuronal network. In addition, ciNs from patients show misfolded protein accumulation and mitochondria ultrastructural abnormalities, biomarkers commonly associated with neurodegeneration. Moreover, for the first time, we show that microfluidic technology, in combination with chemical reprogramming, enables on-chip examination of disease pathological processes and may have important applications in diagnosis. In conclusion, ciNs on microfluidic devices represent a small-scale, non-invasive and cost-effective high-throughput tool for protein misfolding disease diagnosis and may be useful for new biomarker discovery, disease mechanism studies and design of personalised therapies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms23042147Titel-ID: cdi_doaj_primary_oai_doaj_org_article_d8032faf1b0d419a9e23e5babea57fb4
- Format
- –
- Schlagworte
- Abnormalities, Adolescent, Adult, Aged, Aged, 80 and over, Alzheimer Disease - metabolism, Alzheimer Disease - pathology, Alzheimer's disease, Amyloid beta-Peptides - metabolism, Biomarkers, Biomarkers - metabolism, Cerebrospinal fluid, Cognitive Dysfunction - metabolism, Cognitive Dysfunction - pathology, Diagnosis, Direct conversion, Experiments, Female, Fibroblasts, Fibroblasts - metabolism, Fibroblasts - pathology, Humans, induced neurons, Lab-On-A-Chip Devices, Labeling, Magnetic resonance imaging, Male, Medical imaging, Microfluidic devices, microfluidics, Microfluidics - methods, Microscopy, Middle Aged, Mitochondria, Morphology, Neurodegeneration, Neurodegenerative Diseases - metabolism, Neurodegenerative Diseases - pathology, Neuroimaging, Neuroimaging - methods, Neurons, Neurons - metabolism, Neurons - pathology, Neuropathology, Neuropsychological Tests, Parkinson Disease - metabolism, Parkinson Disease - pathology, Parkinson's disease, Pathology, Patients, Positron emission, Positron emission tomography, Protein folding, Proteins, Tomography, transdifferentiation