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Carbon monoxide promotes stomatal initiation by regulating the expression of two EPF genes in Arabidopsis cotyledons
Ist Teil von
Frontiers in plant science, 2022-11, Vol.13, p.1029703-1029703
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2022
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
The gaseous molecule carbon monoxide (CO) can freely pass through the cell membrane and participate in signal transduction in the cell to regulate physiological activities in plants. Here, we report that CO has a positive regulatory role in stomatal development. Exogenous CO donor CORM-2 [Tricarbonyldichlororuthenium (II) dimer] treatment resulted in an increase of stomatal index (SI) on the abaxial epidermis of cotyledons in wild-type, which can be reversed by the addition of the CO biosynthesis inhibitor ZnPPIX [Protoporphyrin IX zinc (II)]. Consistent with this result, mutation of the CO biosynthesis gene
HY1
resulted in a decrease of SI in
hy1-100
plants, while overexpression of
HY1
led to an increase of SI. Further investigation revealed that CO acts upstream of SPCH and YDA in the stomatal development pathway, since the loss of function mutants
spch-1
and
yda-2
were insensitive to CORM-2. The expression of
EPF2
was inhibited by CORM-2 treatment in wild type and is lower in
hy1
than in wild-type plants. In contrast, the expression of
STOMAGEN
was promoted by CORM-2 treatment and is higher in
HY1
-overexpression lines. Loss of function mutants of both
epf2
and
stomagen
are insensitive to CORM-2 treatment. These results indicated that CO positively regulates stomatal initiation and distribution by modulating the expression of
EPF2
and
STOMAGEN
.