Details

Autor(en) / Beteiligte

• Abarca-Zabalía, Judith
• García, Ma Isabel
• Lozano Ros, Alberto
• Marín-Jiménez, Ignacio
• Martínez-Ginés, Maria L
• López-Cauce, Beatriz
• Martín-Barbero, María L
• Salvador-Martín, Sara
• Sanjurjo-Saez, María
• García-Domínguez, Jose M
• López Fernández, Luis A

Titel

Differential Expression of SMAD Genes and S1PR1 on Circulating CD4+ T Cells in Multiple Sclerosis and Crohn's Disease

Ist Teil von

• International journal of molecular sciences, 2020-01, Vol.21 (2), p.676

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The Th17 immune response plays a key role in autoimmune diseases such as multiple sclerosis (MS) and inflammatory bowel disease (IBD). Expression of Th17-related genes in inflamed tissues has been reported in autoimmune diseases. However, values are frequently obtained using invasive methods. We aimed to identify biomarkers of MS in an accessible sample, such as blood, by quantifying the relative expression of 91 Th17-related genes in CD4+ T lymphocytes from patients with MS during a relapse or during a remitting phase. We also compared our findings with those of healthy controls. After confirmation in a validation cohort, expression of and mRNAs was decreased in remitting disease (-2.3-fold and -1.3-fold, respectively) and relapsing disease (-2.2-fold and -1.3-fold, respectively). No differential expression was observed for other SMAD7-related genes, namely, , , and . Under-regulation of and was also observed in another autoimmune disease, Crohn's disease (CD) (-4.6-fold, -1.6-fold, respectively), suggesting the presence of common markers for autoimmune diseases. In addition, expression of , and were also decreased in CD (-2.2-fold, -1.4-fold, -1.6-fold, and -1.6-fold, respectively). Our study suggests that expression of and mRNA in blood samples are markers for MS and CD, and , and for CD. These genes could prove useful as markers of autoimmune diseases, thus obviating the need for invasive methods.