Details

Autor(en) / Beteiligte

• Raza, Hina
• Ashraf, Asmara
• Shamim, Rahat
• Manzoor, Suryyia
• Sohail, Younas
• Khan, Muhammad Imran
• Raza, Nadeem
• Shakeel, Nasir
• Gill, Komal Aziz
• El-Marghany, Adel
• Aftab, Sikandar

Titel

Synthesis and characterization of Hyaluronic Acid (HA) modified polymeric composite for effective treatment of wound healing by transdermal drug delivery system (TDDS)

Ist Teil von

• Scientific reports, 2023-08, Vol.13 (1), p.13425-13425, Article 13425

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Abstract The present study aimed to fabricate a novel polymeric spongy composite to enhance skin regeneration composed of Nystatin (antifungal agent) and Silver Nanoparticles (AgNps). Different formulations (F1–F8) were developed & characterized by using various analytical techniques. AgNps synthesized by chemical reduction method showed spherical morphology 2 µm in size showed by SEM and XRD. A fine porous structure of gel embedded with AgNps having an amorphous structure with 10 % crystallinity due to AgNps was found. IR spectra revealed no chemical interaction between polymers and Nystatin. An increase in thermal stability of formulation was observed till 700 ℃ analyzed by Differential Scanning Calorimetry. Cytotoxic analysis on L929 mouse skin fibroblast cells showed a decrease in cell viability as Ag concentration increased (inactivating Fibroblast and keratinocytes) while 10 mg composition was found safest concentration (94%). Optimized formulation (F2) presented in-vitro drug release up to 90.59% ± 0.76 at pH 7.4, swelling studies (87.5% ± 0.57), water retention (26.60 ± 0.34), pH (5.31 ± 0.03). In the animal burn model, the group that received CHG/Ag/Nystatin healed the wound significantly (p < 0.05). These results suggested that optimized carrier can be used for other anti-fungal drugs facilitating the early healing of the wound.