Details

Autor(en) / Beteiligte

• Sobecki, Michal
• Mrouj, Karim
• Camasses, Alain
• Parisis, Nikolaos
• Nicolas, Emilien
• Llères, David
• Gerbe, François
• Prieto, Susana
• Krasinska, Liliana
• David, Alexandre
• Eguren, Manuel
• Birling, Marie-Christine
• Urbach, Serge
• Hem, Sonia
• Déjardin, Jérôme
• Malumbres, Marcos
• Jay, Philippe
• Dulic, Vjekoslav
• Lafontaine, Denis Lj
• Feil, Robert
• Fisher, Daniel

Titel

The cell proliferation antigen Ki-67 organises heterochromatin

Ist Teil von

• eLife, 2016-03, Vol.5, p.e13722-e13722

Ort / Verlag

England: eLife Science Publications, Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not prevent cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 was relocalised within the nucleus. Finally, overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression.