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Whole-genome sequencing and evolutionary analysis of the wild edible mushroom, Morchella eohespera
Ist Teil von
Frontiers in microbiology, 2024-02, Vol.14, p.1309703-1309703
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2024
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Morels (
, Ascomycota) are an extremely desired group of edible mushrooms with worldwide distribution.
is a typical black morel species, belonging to the Elata clade of
species. The biological and genetic studies of this mushroom are rare, largely hindering the studies of molecular breeding and evolutionary aspects. In this study, we performed
sequencing and assembly of the
strain m200 genome using the third-generation nanopore sequencing platform. The whole-genome size of
was 53.81 Mb with a contig N50 of 1.93 Mb, and the GC content was 47.70%. A total of 9,189 protein-coding genes were annotated. Molecular dating showed that
differentiated from its relative
at ~19.03 Mya (million years ago) in Burdigalian. Evolutionary analysis showed that 657 gene families were contracted and 244 gene families expanded in
versus the related morel species. The non-coding RNA prediction results showed that there were 336 tRNAs, 76 rRNAs, and 45 snRNAs in the
genome. Interestingly, there was a high degree of repetition (20.93%) in the
genome, and the sizes of long interspersed nuclear elements, short interspersed nuclear elements, and long terminal repeats were 0.83 Mb, 0.009 Mb, and 4.56 Mb, respectively. Additionally, selection pressure analysis identified that a total of 492 genes in the
genome have undergone signatures of positive selection. The results of this study provide new insights into the genome evolution of
and lay the foundation for in-depth research into the molecular biology of the genus
in the future.