Details

Autor(en) / Beteiligte

• Enabulele, Egie E
• Le Clec'h, Winka
• Roberts, Emma K
• Thompson, Cody W
• McDonough, Molly M
• Ferguson, Adam W
• Bradley, Robert D
• Anderson, Timothy J C
• Platt, 2nd, Roy N

Titel

Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

Ist Teil von

• Emerging infectious diseases, 2023-08, Vol.29 (8), p.1566-1579

Ort / Verlag

United States: U.S. National Center for Infectious Diseases

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues.