Details

Autor(en) / Beteiligte

• Nakayama, Emi E
• Kubota-Koketsu, Ritsuko
• Sasaki, Tadahiro
• Suzuki, Keita
• Uno, Kazuko
• Shimizu, Jun
• Okamoto, Toru
• Matsumoto, Hisatake
• Matsuura, Hiroshi
• Hashimoto, Shoji
• Tanaka, Toshio
• Harada, Hiromasa
• Tomita, Masafumi
• Kaneko, Mitsunori
• Yoshizaki, Kazuyuki
• Shioda, Tatsuo

Titel

Anti-nucleocapsid antibodies enhance the production of IL-6 induced by SARS-CoV-2 N protein

Ist Teil von

• Scientific reports, 2022-05, Vol.12 (1), p.8108-8108, Article 8108

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• A cytokine storm induces acute respiratory distress syndrome, the main cause of death in coronavirus disease 2019 (COVID-19) patients. However, the detailed mechanisms of cytokine induction due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain unclear. To examine the cytokine production in COVID-19, we mimicked the disease in SARS-CoV-2-infected alveoli by adding the lysate of SARS-CoV-2-infected cells to cultured macrophages or induced pluripotent stem cell-derived myeloid cells. The cells secreted interleukin (IL)-6 after the addition of SARS-CoV-2-infected cell lysate. Screening of 25 SARS-CoV-2 protein-expressing plasmids revealed that the N protein-coding plasmid alone induced IL-6 production. The addition of anti-N antibody further enhanced IL-6 production, but the F(ab') fragment did not. Sera from COVID-19 patients also enhanced IL-6 production, and sera from patients with severer disease induced higher levels of IL-6. These results suggest that anti-N antibody promotes IL-6 production in SARS-CoV-2-infected alveoli, leading to the cytokine storm of COVID-19.