Details

Autor(en) / Beteiligte

• Vidal-Villegas, Beatriz
• Di Pierdomenico, Johnny
• Miralles de Imperial-Ollero, Juan A
• Ortín-Martínez, Arturo
• Nadal-Nicolás, Francisco M
• Bernal-Garro, Jose M
• Cuenca Navarro, Nicolás
• Villegas-Pérez, María P
• Vidal-Sanz, Manuel

Titel

Melanopsin + RGCs Are fully Resistant to NMDA-Induced Excitotoxicity

Ist Teil von

• International journal of molecular sciences, 2019-06, Vol.20 (12), p.3012

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2019

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• We studied short- and long-term effects of intravitreal injection of -methyl-d-aspartate (NMDA) on melanopsin-containing (m ) and non-melanopsin-containing (Brn3a ) retinal ganglion cells (RGCs). In adult SD-rats, the left eye received a single intravitreal injection of 5µL of 100nM NMDA. At 3 and 15 months, retinal thickness was measured in vivo using Spectral Domain-Optical Coherence Tomography (SD-OCT). Ex vivo analyses were done at 3, 7, or 14 days or 15 months after damage. Whole-mounted retinas were immunolabelled for brain-specific homeobox/POU domain protein 3A (Brn3a) and melanopsin (m), the total number of Brn3a RGCs and m RGCs were quantified, and their topography represented. In control retinas, the mean total numbers of Brn3a RGCs and m RGCs were 78,903 ± 3572 and 2358 ± 144 (mean ± SD; = 10), respectively. In the NMDA injected retinas, Brn3a RGCs numbers diminished to 49%, 28%, 24%, and 19%, at 3, 7, 14 days, and 15 months, respectively. There was no further loss between 7 days and 15 months. The number of immunoidentified m RGCs decreased significantly at 3 days, recovered between 3 and 7 days, and were back to normal thereafter. OCT measurements revealed a significant thinning of the left retinas at 3 and 15 months. Intravitreal injections of NMDA induced within a week a rapid loss of 72% of Brn3a RGCs, a transient downregulation of melanopsin expression (but not m RGC death), and a thinning of the inner retinal layers.