eLife, 2018-01, Vol.7
2018
Details
- Autor(en) / Beteiligte
- Titel
- Hsp70-associated chaperones have a critical role in buffering protein production costs
- Ist Teil von
- eLife, 2018-01, Vol.7
- Ort / Verlag
- England: eLife Science Publications, Ltd
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- Proteins are necessary for cellular growth. Concurrently, however, protein production has high energetic demands associated with transcription and translation. Here, we propose that activity of molecular chaperones shape protein burden, that is the fitness costs associated with expression of unneeded proteins. To test this hypothesis, we performed a genome-wide genetic interaction screen in baker's yeast. Impairment of transcription, translation, and protein folding rendered cells hypersensitive to protein burden. Specifically, deletion of specific regulators of the Hsp70-associated chaperone network increased protein burden. In agreement with expectation, temperature stress, increased mistranslation and a chemical misfolding agent all substantially enhanced protein burden. Finally, unneeded protein perturbed interactions between key components of the Hsp70-Hsp90 network involved in folding of native proteins. We conclude that specific chaperones contribute to protein burden. Our work indicates that by minimizing the damaging impact of gratuitous protein overproduction, chaperones enable tolerance to massive changes in genomic expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.29845Titel-ID: cdi_doaj_primary_oai_doaj_org_article_6f8039d030b34e66ac201c61828ad227
- Format
- –
- Schlagworte
- Bacteria, Biology, chaperone overload, Chaperones, Clonal deletion, Colorado, Computational and Systems Biology, Costs, Economic aspects, Energy conservation, Energy Metabolism, Gene expression, Genes, genetic interaction, Genetic screening, Genetics and Genomics, Genomes, Genomics, Germany, Heat shock proteins, Hsp70 protein, HSP72 Heat-Shock Proteins - metabolism, Hsp90 protein, Manufacturing costs, Molecular Chaperones - metabolism, Peptides, Plasmids, protein burden, Protein folding, Proteins, Reproductive fitness, Research centers, Saccharomyces cerevisiae, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - metabolism, Transcription, Transcription (Genetics), Translation