Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Wildlife Is a Potential Source of Human Infections of Enterocytozoon bieneusi and Giardia duodenalis in Southeastern China
Ist Teil von
Frontiers in microbiology, 2021-08, Vol.12, p.692837-692837
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2021
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Wildlife is known to be a source of high-impact pathogens affecting people. However, the distribution, genetic diversity, and zoonotic potential of
Cryptosporidium
,
Enterocytozoon bieneusi
, and
Giardia duodenalis
in wildlife are poorly understood. Here, we conducted the first molecular epidemiological investigation of these three pathogens in wildlife in Zhejiang and Shanghai, China. Genomic DNAs were derived from 182 individual fecal samples from wildlife and then subjected to a nested polymerase chain reaction–based sequencing approach for detection and characterization. Altogether, 3 (1.6%), 21 (11.5%), and 48 (26.4%) specimens tested positive for
Cryptosporidium
species,
E. bieneusi
, and
G. duodenalis
, respectively. Sequence analyses revealed five known (BEB6, D, MJ13, SC02, and type IV) and two novel (designated SH_ch1 and SH_deer1) genotypes of
E. bieneusi
. Phylogenetically, novel
E. bieneusi
genotype SH_deer1 fell into group 6, and the other genotypes were assigned to group 1 with zoonotic potential. Three novel
Cryptosporidium
genotypes (
Cryptosporidium
avian genotype V-like and
C. galli
-like 1 and 2) were identified,
C. galli
-like 1 and 2 formed a clade that was distinct from
Cryptosporidium
species. The genetic distinctiveness of these two novel genotypes suggests that they represent a new species of
Cryptosporidium.
Zoonotic assemblage A (
n
= 36) and host-adapted assemblages C (
n
= 1) and E (
n
= 7) of
G. duodenalis
were characterized. The overall results suggest that wildlife act as host reservoirs carrying zoonotic
E. bieneusi
and
G. duodenalis
, potentially enabling transmission from wildlife to humans and other animals.