Details

Autor(en) / Beteiligte

• Vereide, David T.
• Vickerman, Vernella
• Swanson, Scott A.
• Chu, Li-Fang
• McIntosh, Brian E.
• Thomson, James A.

Titel

An Expandable, Inducible Hemangioblast State Regulated by Fibroblast Growth Factor

Ist Teil von

• Stem cell reports, 2014-12, Vol.3 (6), p.1043-1057

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• During development, the hematopoietic and vascular lineages are thought to descend from common mesodermal progenitors called hemangioblasts. Here we identify six transcription factors, Gata2, Lmo2, Mycn, Pitx2, Sox17, and Tal1, that “trap” murine cells in a proliferative state and endow them with a hemangioblast potential. These “expandable” hemangioblasts (eHBs) are capable, once released from the control of the ectopic factors, to give rise to functional endothelial cells, multilineage hematopoietic cells, and smooth muscle cells. The eHBs can be derived from embryonic stem cells, from fetal liver cells, or poorly from fibroblasts. The eHBs reveal a central role for fibroblast growth factor, which not only promotes their expansion, but also facilitates their ability to give rise to endothelial cells and leukocytes, but not erythrocytes. This study serves as a demonstration that ephemeral progenitor states can be harnessed in vitro, enabling the creation of tractable progenitor cell lines. •Gata2, Lmo2, Mycn, Pitx2, Sox17, and Tal1 induce and maintain a hemangioblast state•FGF2 promotes the expansion of these progenitors and impacts their potency Thomson, Vereide, et al. employ six transcription factors to induce and maintain a hemangioblast state over numerous cell generations in culture. The “expandable hemangioblasts” (eHBs) are controlled by fibroblast growth factor, which stimulates their proliferation and poises the cells to produce endothelial cells and leukocytes.