Details

Autor(en) / Beteiligte

• Bonnet, Céline
• Gou, Panhong
• Girel, Simon
• Bansaye, Vincent
• Lacout, Catherine
• Bailly, Karine
• Schlagetter, Marie-Hélène
• Lauret, Evelyne
• Méléard, Sylvie
• Giraudier, Stéphane

Titel

Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach

Ist Teil von

• iScience, 2021-12, Vol.24 (12), p.103399-103399, Article 103399

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We have reconciled steady-state and stress hematopoiesis in a single mathematical model based on murine in vivo experiments and with a focus on hematopoietic stem and progenitor cells. A phenylhydrazine stress was first applied to mice. A reduced cell number in each progenitor compartment was evidenced during the next 7 days through a drastic level of differentiation without proliferation, followed by a huge proliferative response in all compartments including long-term hematopoietic stem cells, before a return to normal levels. Data analysis led to the addition to the 6-compartment model, of time-dependent regulation that depended indirectly on the compartment sizes. The resulting model was finely calibrated using a stochastic optimization algorithm and could reproduce biological data in silico when applied to different stress conditions (bleeding, chemotherapy, HSC depletion). In conclusion, our multi-step and time-dependent model of immature hematopoiesis provides new avenues to a better understanding of both normal and pathological hematopoiesis. [Display omitted] •We describe a new 6-compartment time-dependent regulated model of hematopoiesis•Biological data under steady state and stress and cell dynamics were used•Modeling is able to recapitulate effects from chemotherapy, bleeding, or HSC depletion Cell biology; Stem cells research; In silico biology