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Higher Prevalence of Bacteroides fragilis in Crohn's Disease Exacerbations and Strain-Dependent Increase of Epithelial Resistance
Ist Teil von
Frontiers in microbiology, 2021-06, Vol.12, p.598232-598232
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2021
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
has previously been linked to Crohn's disease (CD) exacerbations, but results are inconsistent and underlying mechanisms unknown. This study investigates the epidemiology of
and its virulence factors
(enterotoxin) and
among 181 CD patients and the impact on the intestinal epithelial barrier
. The prevalence of
was significantly higher in active (
= 69/88, 78.4%) as compared to remissive (
= 58/93, 62.4%,
= 0.018) CD patients. Moreover,
.
was associated with intestinal strictures. Interestingly, the intestinal barrier function, as examined by transepithelial electrical resistance (TEER) measurements of Caco-2 monolayers, increased when exposed to secretomes of
-positive (
and
isotype; increased TEER ∼160%,
< 0.001) but not when exposed to
negative strains. Whole metagenome sequencing and metabolomics, respectively, identified nine coding sequences and two metabolites that discriminated TEER-increasing from non-TEER-increasing strains. This study revealed a higher
prevalence during exacerbation. Surprisingly,
positive secretomes increased epithelial resistance, but we excluded Bft as the likely causative factor.