Details

Autor(en) / Beteiligte

• de Wit-Verheggen, Vera H W
• Schrauwen-Hinderling, Vera B
• Brouwers, Kim
• Jörgensen, Johanna A
• Schaart, Gert
• Gemmink, Anne
• Nascimento, Emmani B M
• Hesselink, Matthijs K C
• Wildberger, Joachim E
• Segers, Patrique
• Montaigne, David
• Staels, Bart
• Schrauwen, Patrick
• Lindeboom, Lucas
• Hoeks, Joris
• van de Weijer, Tineke

Titel

PCr/ATP ratios and mitochondrial function in the heart. A comparative study in humans

Ist Teil von

• Scientific reports, 2023-05, Vol.13 (1), p.8346-8346, Article 8346

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Cardiac energy status, measured as phosphocreatine (PCr)/adenosine triphosphate (ATP) ratio with 31P-Magnetic Resonance Spectroscopy (31P-MRS) in vivo, is a prognostic factor in heart failure and is lowered in cardiometabolic disease. It has been suggested that, as oxidative phosphorylation is the major contributor to ATP synthesis, PCr/ATP ratio might be a reflection of cardiac mitochondrial function. The objective of the study was to investigate whether PCr/ATP ratios can be used as in vivo marker for cardiac mitochondrial function. We enrolled thirty-eight patients scheduled for open-heart surgery in this study. Cardiac 31P-MRS was performed before surgery. Tissue from the right atrial appendage was obtained during surgery for high-resolution respirometry for the assessment of mitochondrial function. There was no correlation between the PCr/ATP ratio and ADP-stimulated respiration rates (octanoylcarnitine R  < 0.005, p = 0.74; pyruvate R  < 0.025, p = 0.41) nor with maximally uncoupled respiration (octanoylcarnitine R  = 0.005, p = 0.71; pyruvate R  = 0.040, p = 0.26). PCr/ATP ratio did correlate with indexed LV end systolic mass. As no direct correlation between cardiac energy status (PCr/ATP) and mitochondrial function in the heart was found, the study suggests that mitochondrial function might not the only determinant of cardiac energy status. Interpretation should be done in the right context in cardiac metabolic studies.