Details

Autor(en) / Beteiligte

• Song, Hongshuo
• Giorgi, Elena E
• Ganusov, Vitaly V
• Cai, Fangping
• Athreya, Gayathri
• Yoon, Hyejin
• Carja, Oana
• Hora, Bhavna
• Hraber, Peter
• Romero-Severson, Ethan
• Jiang, Chunlai
• Li, Xiaojun
• Wang, Shuyi
• Li, Hui
• Salazar-Gonzalez, Jesus F
• Salazar, Maria G
• Goonetilleke, Nilu
• Keele, Brandon F
• Montefiori, David C
• Cohen, Myron S
• Shaw, George M
• Hahn, Beatrice H
• McMichael, Andrew J
• Haynes, Barton F
• Korber, Bette
• Bhattacharya, Tanmoy
• Gao, Feng

Titel

Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection

Ist Teil von

• Nature communications, 2018-05, Vol.9 (1), p.1928-15, Article 1928

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Recombination in HIV-1 is well documented, but its importance in the low-diversity setting of within-host diversification is less understood. Here we develop a novel computational tool (RAPR (Recombination Analysis PRogram)) to enable a detailed view of in vivo viral recombination during early infection, and we apply it to near-full-length HIV-1 genome sequences from longitudinal samples. Recombinant genomes rapidly replace transmitted/founder (T/F) lineages, with a median half-time of 27 days, increasing the genetic complexity of the viral population. We identify recombination hot and cold spots that differ from those observed in inter-subtype recombinants. Furthermore, RAPR analysis of longitudinal samples from an individual with well-characterized neutralizing antibody responses shows that recombination helps carry forward resistance-conferring mutations in the diversifying quasispecies. These findings provide insight into molecular mechanisms by which viral recombination contributes to HIV-1 persistence and immunopathogenesis and have implications for studies of HIV transmission and evolution in vivo.