Details
- Autor(en) / Beteiligte
- Titel
- The elemental mechanism of transcriptional pausing
- Ist Teil von
- eLife, 2019-01, Vol.8
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Transcriptional pausing underlies regulation of cellular RNA biogenesis. A consensus pause sequence that acts on RNA polymerases (RNAPs) from bacteria to mammals halts RNAP in an elemental paused state from which longer-lived pauses can arise. Although the structural foundations of pauses prolonged by backtracking or nascent RNA hairpins are recognized, the fundamental mechanism of the elemental pause is less well-defined. Here we report a mechanistic dissection that establishes the elemental pause signal ( ) is multipartite; ( ) causes a modest conformational shift that puts γ-proteobacterial RNAP in an off-pathway state in which template base loading but not RNA translocation is inhibited; and ( ) allows RNAP to enter pretranslocated and one-base-pair backtracked states easily even though the half-translocated state observed in paused cryo-EM structures rate-limits pause escape. Our findings provide a mechanistic basis for the elemental pause and a framework to understand how pausing is modulated by sequence, cellular conditions, and regulators.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/eLife.40981Titel-ID: cdi_doaj_primary_oai_doaj_org_article_24639bf2d563493da405524c788c5de8
- Format
- –
- Schlagworte
- Base Pairing - genetics, Base Sequence, Biochemistry and Chemical Biology, Catalysis, Chromosomes and Gene Expression, Consensus Sequence - genetics, Conserved sequence, Deoxyribonucleic acid, DNA, DNA - genetics, Gene expression, Gene regulation, Glycerol, Kinetics, Mutation - genetics, Nucleotide sequence, Nucleotides - metabolism, Ribonucleic acid, RNA, RNA - genetics, RNA polymerase, Templates, Genetic, Transcription, Transcription Elongation, Genetic, Transcription, Genetic