Details
- Autor(en) / Beteiligte
- Titel
- Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans
- Ist Teil von
- eLife, 2018-07, Vol.7
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- During development, neurons form synapses with their fate-determined targets. While we begin to elucidate the mechanisms by which extracellular ligand-receptor interactions enhance synapse specificity by inhibiting synaptogenesis, our knowledge about their intracellular mechanisms remains limited. Here we show that Rap2 GTPase ( ) and its effector, TNIK ( ), act genetically downstream of Plexin ( ) to restrict presynaptic assembly and to form tiled synaptic innervation in . Both constitutively GTP- and GDP-forms of mutants exhibit synaptic tiling defects as mutants, suggesting that cycling of the RAP-2 nucleotide state is critical for synapse inhibition. Consistently, PLX-1 suppresses local RAP-2 activity. Excessive ectopic synapse formation in mutants causes a severe synaptic tiling defect. Conversely, overexpression of strongly inhibited synapse formation, suggesting that is a negative regulator of synapse formation. These results reveal that subcellular regulation of small GTPase activity by Plexin shapes proper synapse patterning in vivo.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.38801Titel-ID: cdi_doaj_primary_oai_doaj_org_article_0faa326f7fa0487893d4bcee6e9a28b0
- Format
- –
- Schlagworte
- Animals, Caenorhabditis elegans - chemistry, Caenorhabditis elegans - genetics, Caenorhabditis elegans Proteins - chemistry, Caenorhabditis elegans Proteins - genetics, Cell adhesion & migration, development, genetics, Guanosine diphosphate, Guanosine Diphosphate - chemistry, Guanosine triphosphatases, Guanosine triphosphate, Guanosine Triphosphate - chemistry, Innervation, Insects, Mammals, Mutation, Nerve Tissue Proteins - chemistry, Nerve Tissue Proteins - genetics, Nervous system, Neurogenesis - genetics, Neurons, Neurons - chemistry, Neuroscience, pattern formation, plexin, Protein-Serine-Threonine Kinases - chemistry, Protein-Serine-Threonine Kinases - genetics, Proteins, rap GTP-Binding Proteins - chemistry, rap GTP-Binding Proteins - genetics, RAP2, Receptors, Cell Surface - chemistry, Receptors, Cell Surface - genetics, Signal Transduction - genetics, synapse, Synapses - chemistry, Synapses - genetics, Synapses - pathology, Synaptogenesis