Details

Autor(en) / Beteiligte

• Frazão, Cláudio J R
• Wagner, Nils
• Rabe, Kenny
• Walther, Thomas

Titel

Construction of a synthetic metabolic pathway for biosynthesis of 2,4-dihydroxybutyric acid from ethylene glycol

Ist Teil von

• Nature communications, 2023-04, Vol.14 (1), p.1931-1931, Article 1931

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Ethylene glycol is an attractive two-carbon alcohol substrate for biochemical product synthesis as it can be derived from CO or syngas at no sacrifice to human food stocks. Here, we disclose a five-step synthetic metabolic pathway enabling the carbon-conserving biosynthesis of the versatile platform molecule 2,4-dihydroxybutyric acid (DHB) from this compound. The linear pathway chains ethylene glycol dehydrogenase, D-threose aldolase, D-threose dehydrogenase, D-threono-1,4-lactonase, D-threonate dehydratase and 2-oxo-4-hydroxybutyrate reductase enzyme activities in succession. We screen candidate enzymes with D-threose dehydrogenase and D-threonate dehydratase activities on cognate substrates with conserved carbon-centre stereochemistry. Lastly, we show the functionality of the pathway by its expression in an Escherichia coli strain and production of 1 g L and 0.8 g L DHB from, respectively, glycolaldehyde or ethylene glycol.