Details

Autor(en) / Beteiligte

• Chabowski, Dawid S
• Kadlec, Andrew O
• Ait-Aissa, Karima
• Hockenberry, Joseph C
• Pearson, Paul J
• Beyer, Andreas M
• Gutterman, David D

Titel

Lysophosphatidic acid acts on LPA 1 receptor to increase H 2 O 2 during flow-induced dilation in human adipose arterioles

Ist Teil von

• British journal of pharmacology, 2018-11, Vol.175 (22), p.4266-4280

Ort / Verlag

England

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• NO produces arteriolar flow-induced dilation (FID) in healthy subjects but is replaced by mitochondria-derived hydrogen peroxide (mtH O ) in patients with coronary artery disease (CAD). Lysophosphatidic acid (LPA) is elevated in patients with risk factors for CAD, but its functional effect in arterioles is unknown. We tested whether elevated LPA changes the mediator of FID from NO to mtH O in human visceral and subcutaneous adipose arterioles. Arterioles were cannulated on glass micropipettes and pressurized to 60 mmHg. We recorded lumen diameter after graded increases in flow in the presence of either NOS inhibition (L-NAME) or H O scavenging (Peg-Cat) ± LPA (10 μM, 30 min), ±LPA /LPA receptor antagonist (Ki16425) or LPA receptor antagonist (H2L5186303). We analysed LPA receptor RNA and protein levels in human arterioles and human cultured endothelial cells. FID was inhibited by L-NAME but not Peg-Cat in untreated vessels. In vessels treated with LPA, FID was of similar magnitude but inhibited by Peg-Cat while L-NAME had no effect. Rotenone attenuated FID in vessels treated with LPA indicating mitochondria as a source of ROS. RNA transcripts from LPA and LPA but not LPA receptors were detected in arterioles. LPA but not LPA receptor protein was detected by Western blot. Pretreatment of vessels with an LPA /LPA , but not LPA , receptor antagonist prior to LPA preserved NO-mediated dilation. These findings suggest an LPA receptor-dependent pathway by which LPA increases arteriolar release of mtH O as a mediator of FMD.