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Theg useg of microorganisms like bacterial in the synthesis of nanoparticles emerges as an eco-friendly approach and an alternative to the chemicals method. In the presentg study, reported the biosynthesis of silver nanoparticles (AgNPs) using the Streptococcus pyogenes. Silver nanoparticles were synthesized through the reduction ofg aqueous Ag + ion using the bacterial culture supernatants at room temperature. Synthesis of AgNPs was initially observed by color change from yellow to brown which was confirmed by UV-visible spectroscopy The silver nanoparticles were further characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopic (SEM) analyses and X_ ray diffraction (XRD). The synthesized nanoparticles were found to be spherical in shape with ag size in the range of 20-100nM. The synthesized AgNPs were found to have antibacterial activity against two tested pathogenic bacteria (Gram negative bacteriag E.coli and Gram positive bacteriag S. aureus.