Details

Autor(en) / Beteiligte

• Kohle, Ferdinand F. E
• Li, Songying
• Turker, Melik Z
• Wiesner, Ulrich B

Titel

Ultrasmall PEGylated and Targeted Core–Shell Silica Nanoparticles Carrying Methylene Blue Photosensitizer

Ist Teil von

• ACS biomaterials science & engineering, 2020-01, Vol.6 (1), p.256-264

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Photodynamic therapy (PDT) presents an alternative noninvasive therapeutic modality for the treatment of cancer and other diseases. PDT relies on cytotoxic singlet oxygen (reactive oxygen species or ROS) that is locally generated through energy transfer between a photosensitizer (PS) and molecularly dissolved triplet oxygen. While a number of nanoparticle-based PS vehicles have been described, because of their beneficial and proven biodistribution and pharmacokinetic profiles, ultrasmall nanoparticles with diameters below 10 nm are particularly promising. Here, we investigate two different particle designs deviating from ultrasmall poly­(ethylene glycol)-coated (PEGylated) fluorescent core–shell silica nanoparticles referred to as Cornell prime dots (C′ dots) by replacing the fluorescent dye with a photosensitizer (psC′ dots), here the methylene blue (MB) derivate MB2. In the first approach (design 1), MB2 is encapsulated into the matrix of the silica core, while in the second approach (design 2), MB2 is grafted onto the silica core surface in between chains of the sterically stabilizing poly­(ethylene glycol) (PEG) corona. We compare both cases with regard to their singlet oxygen quantum yields, ΦΔ, with the effective ΦΔ eff per particle reaching 111 ± 3 and 161 ± 5% for designs 1 and 2, respectively, substantially exceeding single MB2 molecule performance. Encapsulation significantly improves PS photostability, while surface conjugation diminishes it, relative to free MB2. Finally, we show that both particle designs allow functionalization with a targeting peptide, cyclo­(Arg-Gly-Asp-D-Tyr-Cys) [c­(RGDyC)]. Results suggest that psC′ dots are a promising targeted platform for PDT applications, e.g. in oncology, that may combine colloidal stability, efficient renal clearance limiting off-target accumulation, targeted delivery to sites of disease, and effective ROS generation maximizing therapeutic efficacy.