Details

Autor(en) / Beteiligte

• Yuan, Pengfei
• Liu, Litong
• Aipire, Adila
• Zhao, Yanan
• Cai, Shanshan
• Wu, Linjia
• Yang, Xiaofei
• Aimaier, Alimu
• Lu, Jun
• Li, Jinyao

Titel

Evaluation and mechanism of immune enhancement effects of Pleurotus ferulae polysaccharides-gold nanoparticles

Ist Teil von

• International journal of biological macromolecules, 2023-02, Vol.227, p.1015-1026

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• We previously demonstrated that Pleurotus ferulae polysaccharide (PFPS) promoted dendritic cell (DC) maturation through the TLR4 signaling pathway. To improve PFPS activity and bioavailability, gold nanoparticles with PFPS (PFPS-Au NPs) were synthesized. Of note, although the polysaccharide content of PFPS-Au NPs was only one tenth of PFPS, PFPS-Au NPs enhanced the immunostimulatory activities of PFPS in the maturation and function of dendritic cells (DCs) by TLR4 and NLRP3 signaling pathways, evidenced by stronger activation of the down-stream MAPK and NF-κB pathways and NLRP3 inflammasome pathway. More importantly, PFPS-Au NPs enhanced DC migration and murine immunity, particularly in type 1 T-helper cell responses. Moreover, the half-life of PFPS-Au NPs (2.217 ± 0.187 h) was longer than that of PFPS (1.39 ± 0.257 h) in the blood and the distribution of PFPS-Au NPs (19.8 %) in the spleen was significantly increased compared with PFPS (13.3 %), indicating the improved bioavailability in vivo. PFPS-Au NPs as an adjuvant promoted antigen-specific cellular immune responses to an HPV DC-based vaccine, which significantly inhibited the growth of TC-1 tumors in mice. All results suggest that the prepared Au NPs could enhance PFPS-immunostimulatory activity, which will pave the way for PFPS-Au NPs to be applied in clinical trials. •PFPS-AuNP showed higher immunostimulatory activity than PFPS.•PFPS-AuNP promoted DC maturation by TLR4 and NLRP3 signaling pathways.•PFPS-AuNP enhanced the immunity of mice.•The half-life of PFPS-AuNP was longer than that of PFPS in blood of mice.•PFPS-AuNP enhanced immune response and antitumor efficacy of DC-based vaccine.