PloS one, 2014-04, Vol.9 (4), p.e93768-e93768
2014
Details
- Autor(en) / Beteiligte
- Titel
- Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector
- Ist Teil von
- PloS one, 2014-04, Vol.9 (4), p.e93768-e93768
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Tick-borne spotted fever group (SFG) Rickettsia species must be able to infect both vertebrate and arthropod host cells. The host actin-related protein 2/3 (Arp2/3) complex is important in the invasion process and actin-based motility for several intracellular bacteria, including SFG Rickettsia in Drosophila and mammalian cells. To investigate the role of the tick Arp2/3 complex in tick-Rickettsia interactions, open reading frames of all subunits of the protein including Arp2, Arp3, ARPC1, ARPC2, ARPC3, ARPC4, and ARPC5 were identified from Dermacentor variabilis. Amino acid sequence analysis showed variation (ranging from 25-88%) in percent identity compared to the corresponding subunits of the complex from Drosophila melanogaster, Mus musculus, Homo sapiens, and Saccharomyces cerevisiae. Potential ATP binding sites were identified in D. variabilis (Dv) Arp2 and Arp3 subunits as well as five putative WD (Trp-Asp) motifs which were observed in DvARPC1. Transcriptional profiles of all subunits of the DvArp2/3 complex revealed greater mRNA expression in both Rickettsia-infected and -uninfected ovary compared to midgut and salivary glands. In response to R. montanensis infection of the tick ovary, the mRNA level of only DvARPC4 was significantly upregulated compared to uninfected tissues. Arp2/3 complex inhibition bioassays resulted in a decrease in the ability of R. montanensis to invade tick tissues with a significant difference in the tick ovary, indicating a role for the Arp2/3 complex in rickettsial invasion of tick cells. Characterization of tick-derived molecules associated with rickettsial infection is imperative in order to better comprehend the ecology of tick-borne rickettsial diseases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0093768Titel-ID: cdi_plos_journals_1516460104
- Format
- –
- Schlagworte
- Actin, Actin-related protein 2, Actin-Related Protein 2-3 Complex - chemistry, Actin-Related Protein 2-3 Complex - genetics, Actin-Related Protein 2-3 Complex - metabolism, Amino Acid Sequence, Analysis, Animals, Arachnids, Arthropod Vectors - metabolism, Arthropod Vectors - microbiology, Arthropods, Bacteria, Binding Sites, Bioassays, Biology and Life Sciences, Care and treatment, Cloning, Molecular, Comparative analysis, Dermacentor - metabolism, Dermacentor - microbiology, Diagnosis, Drosophila, Ecology, Female, Fever, Fungi, Gene expression, Gene Expression Profiling, Glands, Health aspects, Infections, Insects, Laboratories, Mammalian cells, Medicine and Health Sciences, Midgut, Molecular Sequence Data, Motility, Muscle proteins, Myosin, Open reading frames, Open Reading Frames - genetics, Ovarian cancer, Pathogens, Physiological aspects, Protein Structure, Tertiary, Protein Subunits - chemistry, Protein Subunits - metabolism, Proteins, Rickettsia, Rickettsia Infections - metabolism, Rickettsia Infections - microbiology, Rickettsia rickettsii, Risk factors, RNA, RNA, Messenger - genetics, RNA, Messenger - metabolism, Saccharomyces cerevisiae, Salivary glands, Sequence Alignment, Sequence Analysis, DNA, Tissues, Transcription, Transient receptor potential proteins, Vectors (Biology), Veterinary colleges, Veterinary medicine