Details

Autor(en) / Beteiligte

• Nasrabadi, Ali Mohamadi
• An, Sanggwon
• Kwon, Soon-Bark
• Hwang, Jungho

Titel

Investigation of live and dead status of airborne bacteria using UVAPS with LIVE/DEAD® BacLight Kit

Ist Teil von

• Journal of aerosol science, 2018-01, Vol.115, p.181-189

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2018

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Although the amount of allergens and toxins being originated from viable bacteria is higher than that from dead bacteria, monitoring concentrations of dead bacteria as well as live bacteria is an important issue related to human health. The ultraviolet aerodynamic particle sizer (UVAPS) is an instrument to measure the size distribution of only viable bioaerosols. Therefore, the main objective of this study is to detect percentages of dead as well as live airborne bacteria by using a LIVE/DEAD BacLight Bacterial Viability Kit (L7012, Molecular probes, USA) together with UVAPS. Two different bacteria species, Escherichia coli (E.coli) and Micrococcus luteus (M. luteus), were used for tests. Percentages of fluorescence emitted from these bacteria were compared with those from standard PSL and dust particles. The increased percentages for dead E. coli and dead M. luteus were 41% and 75%, respectively, while this kit did not show any effective influence on fluorescence percentages of polystyrene latex and dust particles. Field test was carried out in various indoor environments to investigate percentages of live and dead airborne bacteria. •The LIVE/DEAD® BacLight Kit makes the UVAPS to be capable to monitor dead bacteria as well as live bioaerosols.•The ratio of live to dead bacteria did not much affect the fluorescence percentage after staining.•Field test was carried out and a correlation was obtained between two fluorescence percentages before and after staining.