Details

Autor(en) / Beteiligte

• Yu, Xiaoyi
• Luo, Yingzi
• Chen, Gangyi
• Liu, Hong
• Tian, Ni
• Zen, Xiaoting
• Huang, Yuting

Titel

Long non-coding RNA PWRN2 regulates cytotoxicity in an in vitro model of age-related macular degeneration

Ist Teil von

• Biochemical and biophysical research communications, 2021-01, Vol.535, p.39-46

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Age-related macular degeneration (AMD) may lead to irreversibly vision loss among aging populations. In this work, in an in vitro AMD cell model, we examined the expression and function of long non-coding RNA, Prader-Willi Region Non-Protein Coding RNA 2 (PWRN2) in injured human retinal pigment epithelial cells. ARPE-19 cell line was maintained in vitro and treated with multi-module stressful conditions, including hydrogen peroxide (H2O2) tert-butylhydroperoxide (t-BuOOH) and ultraviolet B (UVB). Multi-module-stressor-induced cell death was monitored by a viability assay, and PWRN2 expression by qRT-PCR. PWRN2 was either downregulated or upregulated in ARPE-19 cells. The effects of PWRN2 downregulation or upregulation on t-BuOOH-induced cell death, cellular apoptosis and mitochondrial injuries were then quantitatively evaluated. Multi-module stressful conditions induced cell death and PWRN2 upregulation in ARPE-19 cells in vitro. We created ARPE-19 subpopulations with either downregulated or upregulated PWRN2 expressions. Quantitative assays demonstrated that, PWRN2 downregulation effectively alleviated t-BuOOH-induced cell death, apoptosis and various-type of mitochondrial injuries. On the other hand, PWRN2 upregulation worsened t-BuOOH-induced cellular damages in ARPE-19 cells. We demonstrated that downregulating PWRN2 protected multi-module-stressor-induced cell death, apoptosis and mitochondrial injuries in human retinal pigment epithelial cells, suggesting PWRN2 may be an active factor in human AMD. •Multi-module stressful conditions induced cell death, and PWRN2 upregulation in ARPE-19 cells in vitro.•ARPE-19 with either downregulated or upregulated PWRN2 expressions.•PWRN2 downregulation alleviated t-BuOOH -induced cell death in ARPE-19 cells.•PWRN2 downregulation alleviated apoptosis and various-type of mitochondrial injuries.•PWRN2 upregulation worsened t-BuOOH -induced cellular damages in ARPE-19 cells.