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Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state
Ist Teil von
Advances in space research, 2011-03, Vol.47 (6), p.1062-1070
Ort / Verlag
Oxford: Elsevier Ltd
Erscheinungsjahr
2011
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The 53
kDa tumor suppressor protein p53 is generally thought to contribute to the genetic stability of cells and to protect cells from DNA damage through the activity of
p53-centered signal transduction pathways. To clarify the effect of space radiation on the expression of
p53-dependent regulated genes, gene expression profiles were compared between two human cultured lymphoblastoid cell lines: one line (TSCE5) has a wild-type
p53 gene status, and the other line (WTK1) has a mutated
p53 gene status. Frozen human lymphoblastoid cells were stored in a freezer in the International Space Station (ISS) for 133
days. Gene expression was analyzed using DNA chips after culturing the space samples for 6
h on the ground after their return from space. Ground control samples were also cultured for 6
h after being stored in a frozen state on the ground for the same time period that the frozen cells were in space.
p53-Dependent gene expression was calculated from the ratio of the gene expression values in wild-type
p53 cells and in mutated
p53 cells. The expression of 50
p53-dependent genes was up-regulated, and the expression of 94
p53-dependent genes was down-regulated after spaceflight. These expression data identified genes which could be useful in advancing studies in basic space radiation biology. The biological meaning of these results is discussed from the aspect of gene functions in the up- and down-regulated genes after exposure to low doses of space radiation.