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Extraction, Isolation and Characterisation of Antitumor Principle, α-Hederin, from the Seeds of Nigella sativa
Ist Teil von
Planta medica, 2001-02, Vol.67 (1), p.29-32
Ort / Verlag
Stuttgart: Thieme
Erscheinungsjahr
2001
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Abstract
We have previously reported the IN VITRO cytotoxic activity of column fraction 5 (CC-5) of an ethanolic extract of NIGELLA SATIVA seeds. In this study, the effect of CC-5 was evaluated for its IN VIVO antitumor activity against I.P. (intraperitoneally) implanted murine P388 leukemia and S.C. (subcutaneously) implanted LL/2 (Lewis lung carcinoma) cells in BDF1 mice (C57BL/6 × DBA/2 mice). CC-5 at doses of 200 and 400 mg/kg b.w. prolonged the life span of these mice by 153 % compared to DMSO-treated control mice. The antitumor activity of a 21-day treatment of CC-5 against S.C. implanted LL/2 was tested in mice using four experimental protocols as described in the methods. In protocols C and D, CC-5 at a dose of 400 mg/kg b.w. produced significant tumor inhibition rate (TIR) values of 60 % (P < 0.001) and 70 % (P < 0.001) respectively. α-Hederin, a triterpene saponin isolated from CC-5, when given I.P. for 7 days at doses of 5 and 10 mg/kg b.w. to mice with formed tumors, produced significant dose-dependent TIR values of 48 % (P < 0.05) and 65 % (p < 0.01) respectively on day 8 and 50 % (P < 0.01) and 71 % (P < 0.001), respectively, on day 15, compared to 81 % (P < 0.01) on day 8 and 42 % (P < 0.01) on day 15 in the cyclophosphamide (CP)-treated group. The underlying mechanism(s) of antitumor activity of α-hederin remain to be established.