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Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells
Ist Teil von
Journal of biotechnology, 2012-01, Vol.157 (1), p.258-260
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
2012
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
► Here we utilized the synthetic scaffolds strategy to improve resveratrol production in Saccharomyces cerevisiae. ► We observed a 5.0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein. ► This work further demonstrates that synthetic scaffolds can be used for the optimization of engineered metabolic pathways. ► Although the amount of resveratrol produced by the scaffold in Saccharomyces cerevisiae is relatively low when compared to several recent reports on resveratrol engineering, these results demonstrate that the synthetic scaffolds can be applied to resveratrol metabolic engineering to significantly increase the yield. ► When combined with other metabolic engineering methods, the resveratrol production can be further increased.
Resveratrol is a polyphenolic compound produced by a few higher plants when under attack by pathogens such as bacteria or fungi. Besides antioxidant benefits to humans, this health-promoting compound has been reported to extend longevity in yeasts, flies, worms, fishes and obesity mice. Here we utilized the synthetic scaffolds strategy to improve resveratrol production in Saccharomyces cerevisiae. We observed a 5.0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein. This work demonstrated the synthetic scaffolds can be used for the optimization of engineered metabolic pathway.