Details

Autor(en) / Beteiligte

• Folch, Emma
• Serrano, Anna
• Sabater, Lluis
• Gelpí, Emilio
• Roselló-Catafau, Joan
• Closa, Daniel

Titel

Soluble receptors released during acute pancreatitis interfere with the detection of tumor necrosis factor-α

Ist Teil von

• Critical care medicine, 2001-05, Vol.29 (5), p.1023-1026

Ort / Verlag

Hagerstown, MD: by the Society of Critical Care Medicine and Lippincott Williams & Wilkins

Erscheinungsjahr

2001

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• OBJECTIVETo evaluate the interfering effect of tumor necrosis factor-α soluble receptor when measuring circulating concentrations of tumor necrosis factor-α in an experimental model of acute pancreatitis. DESIGNRandomized, controlled trial. SETTINGExperimental laboratory. SUBJECTSMale Wistar rats. INTERVENTIONSAcute pancreatitis was induced by intraductal administration of 5% sodium taurocholate. Saline was administered in a control group. Serums were overloaded with known amounts of tumor necrosis factor-α or macrophage inflammatory protein-2. MEASUREMENTS AND MAIN RESULTS Three hours after induction, serum concentrations of free tumor necrosis factor-α, total tumor necrosis factor-α, and soluble receptor of tumor necrosis factor-α were measured. No detectable concentrations of free tumor necrosis factor-α were found in any experimental group. By contrast, significant increases in total tumor necrosis factor-α and soluble receptor of tumor necrosis factor-α were found after induction of pancreatitis. Overloading of serum with tumor necrosis factor-α resulted in detection of 50% of the expected concentrations of free tumor necrosis factor-α from control animals and only of 5% from the pancreatitis group. Overloading the serum with macrophage inflammatory protein-2 resulted in a detection of 100% of the expected concentrations in both control and treated animals. CONCLUSIONCirculating soluble receptor of tumor necrosis factor-α could interfere with the detection of tumor necrosis factor-α in some pathologies, such as pancreatitis, that are associated with increases in soluble receptor of tumor necrosis factor-α.