Details

Autor(en) / Beteiligte

• Liu, Wei
• Wacker, Daniel
• Gati, Cornelius
• Han, Gye Won
• James, Daniel
• Wang, Dingjie
• Nelson, Garrett
• Weierstall, Uwe
• Katritch, Vsevolod
• Barty, Anton
• Zatsepin, Nadia A.
• Li, Dianfan
• Messerschmidt, Marc
• Boutet, Sébastien
• Williams, Garth J.
• Koglin, Jason E.
• Seibert, M. Marvin
• Wang, Chong
• Shah, Syed T. A.
• Basu, Shibom
• Fromme, Raimund
• Kupitz, Christopher
• Rendek, Kimberley N.
• Grotjohann, Ingo
• Fromme, Petra
• Kirian, Richard A.
• Beyerlein, Kenneth R.
• White, Thomas A.
• Chapman, Henry N.
• Caffrey, Martin
• Spence, John C. H.
• Stevens, Raymond C.
• Cherezov, Vadim

Titel

Serial Femtosecond Crystallography of G Protein-Coupled Receptors

Ist Teil von

• Science (American Association for the Advancement of Science), 2013-12, Vol.342 (6165), p.1521-1524

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2013

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidie cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.