Details

Autor(en) / Beteiligte

• Vradi, Linda
• Gray, Mark
• Groundwater, Paul W
• Hall, Andrew J
• James, Arthur L
• Orenga, Sylvain
• Perry, John D
• Anderson, Rosaleen J

Titel

Synthesis and evaluation of fluorogenic 2-amino-1,8-naphthyridine derivatives for the detection of bacteriaElectronic supplementary information (ESI) available. See DOI: 10.1039/c2ob06986e

Erscheinungsjahr

2012-04

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Several novel fluorogenic N -aminoacylnaphthyridine substrates were synthesized in good yield and tested for their ability to detect pathogenic bacteria in agar-based cell culture. Simple 2- N -(-alanyl)amino-5,7-dialkylnaphthyridine substrates were selectively hydrolysed by -alanylaminopeptidase expressing bacteria, but were subject to diffusion in the agar medium. Diffusion was reduced in the 2- N -(-alanyl)amino-7-alkylnaphthyridine substrates with longer alkyl chains, but inhibition of growth was increased. 2- N -(-Alanyl)amino-7-octylnaphthyridine inhibited the growth of all species tested, except for strains resistant to colistin/polymyxin, providing a rationale for the development of substrates for the selective detection of drug resistant species in clinical samples. Novel 2- N -(-alanyl)amino-1,8-aminonaphthyridines were hydrolysed by specific enzymatic activity, resulting in visible fluorescence and the selective detection of pathogenic bacteria.