Details

Autor(en) / Beteiligte

• Deng, Cai-Yun
• Lei, Wen-Liang
• Xu, Xiao-Hui
• Ju, Xiang-Chun
• Liu, Yang
• Luo, Zhen-Ge

Titel

JIP1 mediates anterograde transport of Rab10 cargos during neuronal polarization

Ist Teil von

• The Journal of neuroscience, 2014-01, Vol.34 (5), p.1710-1723

Ort / Verlag

United States: Society for Neuroscience

Erscheinungsjahr

2014

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Axon development and elongation require strictly controlled new membrane addition. Previously, we have shown the involvement of Rab10 in directional membrane insertion of plasmalemmal precursor vesicles (PPVs) during neuronal polarization and axonal growth. However, the mechanism responsible for PPV transportation remains unclear. Here we show that c-Jun N-terminal kinase-interacting protein 1 (JIP1) interacts with GTP-locked active form of Rab10 and directly connects Rab10 to kinesin-1 light chain (KLC). The kinesin-1/JIP1/Rab10 complex is required for anterograde transport of PPVs during axonal growth. Downregulation of JIP1 or KLC or disrupting the formation of this complex reduces anterograde transport of PPVs in developing axons and causes neuronal polarity defect. Furthermore, this complex plays an important role in neocortical neuronal polarization of rats in vivo. Thus, this study has demonstrated a mechanism underlying directional membrane trafficking involved in axon development.