Molecular biology of the cell, 2019-06, Vol.30 (12), p.1477-1489
2019
Details
- Autor(en) / Beteiligte
- Titel
- Arf6, JIP3, and dynein shape and mediate macropinocytosis
- Ist Teil von
- Molecular biology of the cell, 2019-06, Vol.30 (12), p.1477-1489
- Ort / Verlag
- United States: The American Society for Cell Biology
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Macropinocytosis is an actin-driven form of clathrin-independent endocytosis that generates an enlarged structure, the macropinosome. Although many studies focus on signaling molecules and phosphoinositides involved in initiating macropinocytosis, the commitment to forming a macropinosome and the handling of that membrane have not been studied in detail. Here we show in HT1080 cells, a human fibrosarcoma cell line, a requirement for microtubules, dynein, the JIP3 microtubule motor scaffold protein, and Arf6, a JIP3 interacting protein, for the formation and inward movement of the macropinosome. While actin and myosin II also play critical roles in the formation of ruffling membrane, microtubules provide an important tract for initiation, sealing, and transport of the macropinosome through the actin- and myosin-rich lamellar region.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1059-1524eISSN: 1939-4586DOI: 10.1091/mbc.e19-01-0022Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6724687
- Format
- –
- Schlagworte
- Actins - metabolism, Adaptor Proteins, Signal Transducing - metabolism, ADP-Ribosylation Factors - metabolism, Cell Line, Tumor, Clathrin - metabolism, Dyneins - metabolism, Humans, Microtubules - metabolism, Models, Biological, Mutation - genetics, Nerve Tissue Proteins - metabolism, Pinocytosis