The Journal of experimental medicine, 2018-07, Vol.215 (7), p.1869-1890
2018
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8 + T cells
- Ist Teil von
- The Journal of experimental medicine, 2018-07, Vol.215 (7), p.1869-1890
- Ort / Verlag
- United States: Rockefeller University Press
- Erscheinungsjahr
- 2018
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- T cells are actively scanning pMHC-presenting cells in lymphoid organs and nonlymphoid tissues (NLTs) with divergent topologies and confinement. How the T cell actomyosin cytoskeleton facilitates this task in distinct environments is incompletely understood. Here, we show that lack of Myosin IXb (Myo9b), a negative regulator of the small GTPase Rho, led to increased Rho-GTP levels and cell surface stiffness in primary T cells. Nonetheless, intravital imaging revealed robust motility of Myo9b CD8 T cells in lymphoid tissue and similar expansion and differentiation during immune responses. In contrast, accumulation of Myo9b CD8 T cells in NLTs was strongly impaired. Specifically, Myo9b was required for T cell crossing of basement membranes, such as those which are present between dermis and epidermis. As consequence, Myo9b CD8 T cells showed impaired control of skin infections. In sum, we show that Myo9b is critical for the CD8 T cell adaptation from lymphoid to NLT surveillance and the establishment of protective tissue-resident T cell populations.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1007eISSN: 1540-9538DOI: 10.1084/jem.20170896Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6028505
- Format
- –
- Schlagworte
- Actomyosin, Adaptive immunity, Animals, Basement membranes, CD8 antigen, CD8-Positive T-Lymphocytes - cytology, CD8-Positive T-Lymphocytes - metabolism, Cell Movement, Cell Polarity, Cell Proliferation, Cell surface, Cells, Cultured, Cytoskeleton, Dermis, Disease Models, Animal, Epidermis, Epidermis - pathology, Epidermis - virology, Extracellular Matrix - metabolism, Guanosine triphosphatases, Guanosine triphosphate, Immune response, Immunity, Infiltration, Lymphocyte Activation - immunology, Lymphocytes, Lymphocytes T, Lymphoid tissue, Lymphoid Tissue - metabolism, Membranes, Mice, Inbred C57BL, Motility, Myosin, Myosins - deficiency, Myosins - metabolism, Organs, Receptors, Lymphocyte Homing - metabolism, rho GTP-Binding Proteins - metabolism, Rodents, Skin, Stiffness, Tissues