Details

Autor(en) / Beteiligte

• Mukhopadhya, Anindya
• Noronha, Nessa
• Bahar, Bojlul
• Ryan, Marion T.
• Murray, Brian A.
• Kelly, Phil M.
• O'Loughlin, Ian B.
• O'Doherty, John V.
• Sweeney, Torres

Titel

Anti‐inflammatory effects of a casein hydrolysate and its peptide‐enriched fractions on TNFα‐challenged Caco‐2 cells and LPS‐challenged porcine colonic explants

Ist Teil von

• Food science & nutrition, 2014-11, Vol.2 (6), p.712-723

Ort / Verlag

United States: John Wiley & Sons, Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti‐inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide‐enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco‐2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco‐2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co‐treated with casein hydrolysates for 24 h. Following this, interleukin (IL)‐8 concentrations in the supernatant were measured using enzyme‐linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co‐treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL‐8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL‐8 by 68.7% and 66.1%, respectively, relative to TNFα‐stimulated Caco‐2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease in IL1‐α, IL1‐β, IL‐8, TGF‐β and IL‐10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti‐inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti‐inflammatory properties of the 1 kDaR is proposed. Bioactive milk peptides derived from milk proteins illicit a wide range of physiological benefits. The potent bioactive peptides present in the 1 kDaR fraction of the sodium caseinate hydrolysate, used in this study, was associated with anti‐inflammatory activity and may serve as an active functional food ingredient.