Details

Autor(en) / Beteiligte

• Benedetti, Fabrizio
• Dorier, Julien
• Stasiak, Andrzej

Titel

Effects of supercoiling on enhancer-promoter contacts

Ist Teil von

• Nucleic acids research, 2014-09, Vol.42 (16), p.10425

Ort / Verlag

England

Erscheinungsjahr

2014

Link zum Volltext

Beschreibungen/Notizen

• Using Brownian dynamics simulations, we investigate here one of possible roles of supercoiling within topological domains constituting interphase chromosomes of higher eukaryotes. We analysed how supercoiling affects the interaction between enhancers and promoters that are located in the same or in neighbouring topological domains. We show here that enhancer-promoter affinity and supercoiling act synergistically in increasing the fraction of time during which enhancer and promoter stay in contact. This stabilizing effect of supercoiling only acts on enhancers and promoters located in the same topological domain. We propose that the primary role of recently observed supercoiling of topological domains in interphase chromosomes of higher eukaryotes is to assure that enhancers contact almost exclusively their cognate promoters located in the same topological domain and avoid contacts with very similar promoters but located in neighbouring topological domains.