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BibTeX
Proteomic profiling of halloysite clay nanotube exposure in intestinal cell co-culture
Journal of applied toxicology, 2013-11, Vol.33 (11), p.1316-1329
Lai, Xianyin
Agarwal, Mangilal
Lvov, Yuri M.
Pachpande, Chetan
Varahramyan, Kody
Witzmann, Frank A.
2013
Details
Autor(en) / Beteiligte
Lai, Xianyin
Agarwal, Mangilal
Lvov, Yuri M.
Pachpande, Chetan
Varahramyan, Kody
Witzmann, Frank A.
Titel
Proteomic profiling of halloysite clay nanotube exposure in intestinal cell co-culture
Ist Teil von
Journal of applied toxicology, 2013-11, Vol.33 (11), p.1316-1329
Ort / Verlag
England: Blackwell Publishing Ltd
Erscheinungsjahr
2013
Link zum Volltext
Quelle
Wiley Online Library
Beschreibungen/Notizen
ABSTRACT Halloysite is aluminosilicate clay with a hollow tubular structure with nanoscale internal and external diameters. Assessment of halloysite biocompatibility has gained importance in view of its potential application in oral drug delivery. To investigate the effect of halloysite nanotubes on an in vitro model of the large intestine, Caco‐2/HT29‐MTX cells in monolayer co‐culture were exposed to nanotubes for toxicity tests and proteomic analysis. Results indicate that halloysite exhibits a high degree of biocompatibility characterized by an absence of cytotoxicity, in spite of elevated pro‐inflammatory cytokine release. Exposure‐specific changes in expression were observed among 4081 proteins analyzed. Bioinformatic analysis of differentially expressed protein profiles suggest that halloysite stimulates processes related to cell growth and proliferation, subtle responses to cell infection, irritation and injury, enhanced antioxidant capability, and an overall adaptive response to exposure. These potentially relevant functional effects warrant further investigation in in vivo models and suggest that chronic or bolus occupational exposure to halloysite nanotubes may have unintended outcomes. Copyright © 2013 John Wiley & Sons, Ltd. In view of their potential application in oral drug delivery, we investigated the effect of halloysite nanotubes in an in vitro model of the large intestine. Toxicity tests and label‐free quantitative mass spectrometric analysis indicate that halloysite exhibits a high degree of biocompatibility characterized by an absence of cytotoxicity, despite elevated proinflammatory cytokine release. Bioinformatic analysis of differentially expressed protein profiles suggest that halloysite stimulates processes related to cell growth and proliferation, subtle responses to cell infection, irritation and injury, enhanced antioxidant capability, and an overall adaptive response to exposure.
Sprache
Englisch
Identifikatoren
ISSN: 0260-437X
eISSN: 1099-1263
DOI: 10.1002/jat.2858
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3789846
Format
–
Schlagworte
Aluminum Silicates - chemistry
,
Aluminum Silicates - toxicity
,
Biocompatibility
,
Caco-2 Cells
,
Cell culture
,
Cell Membrane Permeability - drug effects
,
Cell Survival - drug effects
,
Coculture Techniques
,
Drug Carriers - chemistry
,
Drug Carriers - toxicity
,
Drug delivery systems
,
enterocytes
,
halloysite
,
HT29 Cells
,
Humans
,
intestinal epithelia
,
Intestines - drug effects
,
Intestines - metabolism
,
label-free quantitative mass spectrometry
,
Large intestine
,
Microscopy, Electron, Transmission
,
Nanocomposites
,
Nanomaterials
,
Nanostructure
,
Nanotubes
,
Nanotubes - chemistry
,
Nanotubes - toxicity
,
Particle Size
,
Proteins
,
Proteome - metabolism
,
Proteomics
,
Surface Properties
,
Toxicity
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