EMBO reports, 2011-07, Vol.12 (7), p.697-704
2011
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Details
- Autor(en) / Beteiligte
- Titel
- G-quadruplex RNA structure as a signal for neurite mRNA targeting
- Ist Teil von
- EMBO reports, 2011-07, Vol.12 (7), p.697-704
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 2011
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis‐acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best‐known dendritic mRNAs contain a guanine (G)–quadruplex consensus in their 3′‐untranslated region. Among these mRNAs, we show by using RNA structure probing that a G–quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD‐95 and CaMKIIa. The G–quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor‐responsive manner. Thus, G–quadruplex seems to be a common neurite localization signal. Guanine‐quadruplex structures in the 3'‐UTR of neuronal mRNAs are shown to function as a neurite mRNA localization signal in mouse cortical neurons in a metabotrobic glutamate receptor‐responsive manner.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1469-221XeISSN: 1469-3178DOI: 10.1038/embor.2011.76Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3128965
- Format
- –
- Schlagworte
- 3' Untranslated regions, Animals, Axon guidance, Axons, Base Sequence, Biochemistry, Molecular Biology, Ca super(2+)/calmodulin-dependent protein kinase II, Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics, CaMKIIa, Disks Large Homolog 4 Protein, FMRP, G-Quadruplexes, Glutamic acid, Glutamic acid receptors (metabotropic), Guanine, Guanine-quadruplex, Humans, Intracellular Signaling Peptides and Proteins - genetics, Life Sciences, Membrane Proteins - genetics, Mice, Mice, Inbred C57BL, Molecular biology, Molecular Sequence Data, mRNA, mRNA trafficking, Neurites - metabolism, Neurons, Nucleic Acid Conformation, Postsynaptic density proteins, Proteins, PSD-95, Regulatory sequences, Ribonucleic acid, RNA, RNA Transport, RNA, Messenger - chemistry, RNA, Messenger - metabolism, Scientific Reports, Signal transduction