Details

Autor(en) / Beteiligte

• JACKSON, N
• LOWE, J
• BALL, J
• BROMIDGE, E
• LING, N. R
• LARKINS, S
• GRIFFITH, M. J
• FRANKLIN, I. M

Titel

Two new IgA1-κ plasma cell leukaemia cell lines (JJN-1 & JJN-2) which proliferate in response to B cell stimulatory factor 2

Ist Teil von

• Clinical and experimental immunology, 1989, Vol.75 (1), p.93-99

Ort / Verlag

Oxford: Blackwell

Erscheinungsjahr

1989

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Two new cell lines with the phenotype of terminally differentiated B cells have been derived from the presentation bone marrow of a patient with plasma cell leukaemia. They express the same immunoglobulin (A1-kappa) as the original bone marrow cells. JJN-1 is an hypodiploid, slow-growing line with a plasmacytic morphology, which grows in medium with 15-20% fetal calf serum. When JJN-1 was stimulated with a supernatant ('ESG') containing B cell stimulatory factor 2 (BSF-2/IL-6), a hypotetraploid sub-line, JJN-2, was selectively stimulated. JJN-2 is dependent on ESG for survival. The stimulatory effect of ESG can be completely abrogated by an anti-BSF-2 monoclonal antibody. However, purified BSF-2 alone only produces sub-maximal stimulation of the lines. Both lines show complex karyotypic abnormalities, including 14q- and del(6q). JJN-1 and JJN-2 may be useful for the study of late B cell differentiation and for use as immunogens for the generation of anti-plasma cell monoclonal antibodies.