Details

Autor(en) / Beteiligte

• Seim, Roland F.
• Glinski, Donna A.
• Lavelle, Candice M.
• Awkerman, Jill A.
• Hemmer, Becky L.
• Harris, Peggy
• Raimondo, Sandy
• Snyder, Marcía N.
• Acrey, Brad W.
• Purucker, S. Thomas
• MacMillan, Denise K.
• Brennan, Amanda A.
• Henderson, W. Matthew

Titel

Using metabolomic profiling to inform use of surrogate species in ecological risk assessment practices

Ist Teil von

• Comparative biochemistry and physiology. Part D, Genomics & proteomics, 2022-03, Vol.41 (C), p.100947-100947, Article 100947

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

ScienceDirect Journals (5 years ago - present)

Beschreibungen/Notizen

• The U.S. EPA frequently uses avian or fish toxicity data to set protective standards for amphibians in ecological risk assessments. However, this approach does not always adequately represent aquatic-dwelling and terrestrial-phase amphibian exposure data. For instance, it is accepted that early life stage tests for fish are typically sensitive enough to protect larval amphibians, however, metamorphosis from tadpole to a terrestrial-phase adult relies on endocrine cues that are less prevalent in fish but essential for amphibian life stage transitions. These differences suggest that more robust approaches are needed to adequately elucidate the impacts of pesticide exposure in amphibians across critical life stages. Therefore, in the current study, methodology is presented that can be applied to link the perturbations in the metabolomic response of larval zebrafish (Danio rerio), a surrogate species frequently used in ecotoxicological studies, to those of African clawed frog (Xenopus laevis) tadpoles following exposure to three high-use pesticides, bifenthrin, chlorothalonil, or trifluralin. Generally, D. rerio exhibited greater metabolic perturbations in both number and magnitude across the pesticide exposures as opposed to X. laevis. This suggests that screening ecological risk assessment surrogate toxicity data would sufficiently protect amphibians at the single life stage studied but care needs to be taken to understand the suite of metabolic requirements of each developing species. Ultimately, methodology presented, and data gathered herein will help inform the applicability of metabolomic profiling in establishing the risk pesticide exposure poses to amphibians and potentially other non-target species. [Display omitted] •Larval D. rerio and X. laevis tadpoles were acutely exposed to high-use pesticides.•Metabolomic profiling was used to identify pesticide-perturbed metabolites.•Larval D. rerio elicited a more perturbed metabolic response than X. laevis tadpoles.•Cellular processes and functions are impacted in both species following exposures.•Variously affected pathways can result in protective acute fish surrogacy for tadpoles.