Details
- Autor(en) / Beteiligte
- Titel
- Essential requirement for JPT2 in NAADP-evoked Ca 2+ signaling
- Ist Teil von
- Science signaling, 2021-03, Vol.14 (675)
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Beschreibungen/Notizen
- Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a "clickable" NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca signaling and control of coronaviral entry.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1937-9145DOI: 10.1126/scisignal.abd5605Titel-ID: cdi_pubmed_primary_33758061
- Format
- –
- Schlagworte
- Affinity Labels, Animals, Calcium Channels - metabolism, Calcium Signaling - physiology, Carrier Proteins - metabolism, Click Chemistry - methods, COVID-19 - metabolism, COVID-19 - virology, Gene Knockdown Techniques, HEK293 Cells, Humans, Microtubule-Associated Proteins - antagonists & inhibitors, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, NADP - analogs & derivatives, NADP - metabolism, Recombinant Proteins - genetics, Recombinant Proteins - metabolism, SARS-CoV-2 - physiology, Second Messenger Systems - physiology, Transcriptome, Virus Internalization