Details

Autor(en) / Beteiligte

• Christensen, Idd A
• Eijsink, Vincent G H
• Aachmann, Finn L
• Courtade, Gaston

Titel

1 H, 13 C, 15 N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10A from Jonesia denitrificans

Ist Teil von

• Biomolecular NMR assignments, 2021-04, Vol.15 (1), p.79

Ort / Verlag

Netherlands

Erscheinungsjahr

2021

Link zum Volltext

Quelle

SpringerLink Journals

Beschreibungen/Notizen

• The lytic polysaccharide monooxygenase JdLPMO10A is the N-terminal domain of the multimodular protein Jd1381. The isolated JdLPMO10A domain is one of the smallest chitin-active lytic polysaccharide monooxygenases known to date with a size of only 15.5 kDa. JdLPMO10A is a copper-dependent oxidative enzyme that depolymerizes chitin by hydroxylating the C1 carbon in the glycosidic bond. JdLPMO10A has been isotopically labeled and recombinantly expressed. Here, we report the H, C, N resonance assignment of JdLPMO10A. Secondary structural elements predicted based on the NMR assignment are in excellent agreement with the crystal structure of JdLPMO10A.