Details

Autor(en) / Beteiligte

• Sá, Chayenne Alves
• Vieira, Leonardo Rogério
• Pereira Almeida Filho, Luiz Carlos
• Real-Guerra, Rafael
• Lopes, Fernanda Cortez
• Souza, Terezinha Maria
• Vasconcelos, Ilka Maria
• Staniscuaski, Fernanda
• Carlini, Célia Regina
• Urano Carvalho, Ana Fontenele
• Farias, Davi Felipe

Titel

Risk assessment of the antifungal and insecticidal peptide Jaburetox and its parental protein the Jack bean (Canavalia ensiformis) urease

Ist Teil von

• Food and chemical toxicology, 2020-02, Vol.136, p.110977, Article 110977

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Jaburetox (JBTX) is an insecticidal and antifungal peptide derived from jack bean (Canavalia ensiformis) urease that has been considered a candidate for developing genetically modified crops. This study aimed to perform the risk assessment of the peptide JBTX following the general recommendations of the two-tiered, weight-of-evidence approach proposed by International Life Sciences Institute. The urease of C. ensiformis (JBU) and its isoform JBURE IIb (the JBTX parental protein) were assessed. The history of safe use revealed no hazard reports for the studied proteins. The available information shows that JBTX possesses selective activity against insects and fungi. JBTX and JBU primary amino acids sequences showed no relevant similarity to toxic, antinutritional or allergenic proteins. Additionally, JBTX and JBU were susceptible to in vitro digestibility, and JBU was also susceptible to heat treatment. The results did not identify potential risks of adverse effects and reactions associated to JBTX. However, further allergen (e.g. serum IgE binding test) and toxicity (e.g. rodent toxicity tests) experimentation can be done to gather additional safety information on JBTX, and to meet regulatory inquiries for commercial approval of transgenic cultivars expressing this peptide. [Display omitted] •Jaburetox is an antifungal and insecticidal peptide derived from jack bean urease.•Jaburetox possesses selective activity against insects and fungi.•Jaburetox showed no significant similarity to any allergenic protein.•Jaburetox was degraded in simulated gastric fluid containing pepsin.•This study did not identify potential risks associated with Jaburetox.