Proceedings of the National Academy of Sciences - PNAS, 1988-10, Vol.85 (19), p.7211-7215
1988
Details
- Autor(en) / Beteiligte
- Titel
- Structural Analysis of Complementary DNA and Amino Acid Sequences of Human and Rat Androgen Receptors
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1988-10, Vol.85 (19), p.7211-7215
- Ort / Verlag
- United States: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1988
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Structural analysis of cDNAs for human and rat androgen receptors (ARs) indicates that the amino-terminal regions of ARs are rich in oligo- and poly(amino acid) motifs as in some homeotic genes. The human AR has a long stretch of repeated glycines, whereas rat AR has a long stretch of glutamines. There is a considerable sequence similarity among ARs and the receptors for glucocorticoids, progestins, and mineralocorticoids within the steroid-binding domains. The cysteine-rich DNA-binding domains are well conserved. Translation of mRNA transcribed from AR cDNAs yielded 94- and 76-kDa proteins and smaller forms that bind to DNA and have high affinity toward androgens. These rat or human ARs were recognized by human autoantibodies to natural ARs. Molecular hybridization studies, using AR cDNAs as probes, indicated that the ventral prostate and other male accessory organs are rich in AR mRNA and that the production of AR mRNA in the target organs may be autoregulated by androgens.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.85.19.7211Titel-ID: cdi_pubmed_primary_3174628
- Format
- –
- Schlagworte
- 550201 - Biochemistry- Tracer Techniques, ADRENAL HORMONES, AMINO ACID SEQUENCE, AMINO ACIDS, ANDROGENS, ANDROSTANES, ANIMALS, Base Sequence, BASIC BIOLOGICAL SCIENCES, BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, Binding Sites, CARBOXYLIC ACIDS, Cogs, Complementary DNA, CORTICOSTEROIDS, CYSTEINE, DAYS LIVING RADIOISOTOPES, DNA, DNA - analysis, DRUGS, ELECTROPHORESIS, EVEN-ODD NUCLEI, GENES, Gin, GLUCOCORTICOIDS, HORMONES, Humans, HYBRIDIZATION, HYDROGEN COMPOUNDS, HYDROXY COMPOUNDS, ISOTOPES, KETONES, LIGHT NUCLEI, LIPOTROPIC FACTORS, MAMMALS, MAN, MEMBRANE PROTEINS, MESSENGER-RNA, METHIONINE, Molecular Sequence Data, MOLECULAR STRUCTURE, NUCLEI, NUCLEIC ACIDS, Nucleotides, ORGANIC ACIDS, ORGANIC COMPOUNDS, ORGANIC SULFUR COMPOUNDS, PREGNANES, PRIMATES, Prostate, Protein Biosynthesis, PROTEINS, RADIOISOTOPES, RATS, RECEPTORS, Receptors, Androgen - analysis, Receptors, Androgen - genetics, RECOMBINANT DNA, RNA, RODENTS, STEROID HORMONES, Steroid receptors, STEROIDS, SULFUR 35, SULFUR ISOTOPES, THIOLS, Transcription, Genetic, TRITIUM COMPOUNDS, VERTEBRATES